Age-related osteogenesis on lateral force application to rat incisor – Part I: Premaxilla suture remodeling

Abstract

Objectives: The suture is a fibrous tissue intervening two adjacent bone segments, existing only in the craniofacial region. In spite of wide use of palatal expansion in various ages, the age-dependent cellular mechanism for osteogenesis is largely unknown. The aim of this study was to examine the proliferation and differentiation pattern of the suture cells on lateral expansion in rats depending on the ages. Materials and Methods: Calibrated lateral tensile stress of 50 g was given to the male Sprague-Dawley rat incisors using a double helix in 30 young (10 weeks) and another 30 aged (52 weeks) group, respectively. Each group was subdivided into control, 1, 3, 7, 14, and 21 days, with five animals in each group. Premaxilla area was retrieved from each animal for further histologic analyses including H and E, Masson’s trichrome, and immunohistochemical staining using antibodies against phospho-extracellular signal-regulated kinase, proliferating cell nuclear antigen (PCNA), and fibroblast growth factor receptor-2 (FGFR2). Positive cell counts in the region of interest were conducted. Results: Gross suture separation and subsequent bone formation on the sutural side bone surface were observed in both groups, characterized as active collagen turnover, remarkable woven bone projection toward the sutural mesenchyme and subsequent maturation in 3 weeks. Increase in PCNA- and FGFR2-postive cell proportions were comparable in both groups, indicating similar time- and area-specific proliferation and osteogenic differentiation patterns in the stretched suture regardless of the age groups. Conclusion: According to the results, it can be implicated that the tensile stress applied to the suture in the adult group may induce active bone formation similar to that in young group, in associated with FGFR2 and Erk signaling cascade. Mesenchymal cells in the premaxillary suture appear to retain remarkable potential for further proliferation and differentiation even in aged subjects.