Genetic polymorphism of interleukin-12β (rs7709212) in chronic and aggressive periodontitis in a group of the Bengali population of India

Abstract

Objectives: The genetic basis of periodontitis was demonstrated by formal genetic studies which were focused on a range of various candidate genes selected for their roles in the immune system like genes of the interleukins (IL). Bone proresorptive cytokines such as IL-1 and tumor necrosis factor-α are primarily regulated by a network of other T-cell derived cytokines, including IL-12 family. Specific objective of this study was to find out any association between IL-12β 3΄ untranslated region (UTR) (rs7709212) gene polymorphism and chronic as well as aggressive periodontitis (AgP) in Bengali population of West Bengal, India. Materials and Methods: A total of 88 Bengali participants of both sexes were recruited and were divided into three groups: Group A (chronic periodontitis [CP] group), Group B (AgP group), and Group C (healthy control). DNA extraction from collected peripheral venous blood was performed by phenol chloroform method and ethanol precipitation. Genotyping of extracted DNA samples was carried out for locus IL-12β 3΄UTR (rs7709212) by real time-polymerase chain reaction. Association between genotypes and cases was examined by the odds ratio with 95% confidence interval and chi-square analysis using R statistical software. Statistical significance was defined as P < 0.05. Results: On analysis of the minor allele frequencies of total periodontitis cases and control, the results were found to be statistically significant with the P = 0.03045. Conclusion: The present study suggested a strong association of single nucleotide polymorphism of IL-12β (rs7709212) with total periodontitis cases (both CP and AgP) in the present study cohort.