Enzyme-Linked Immunosorbent Assay versus Chemiluminescent Immunoassay: A General Overview-Reference-Cited by-同舟云学术

Enzyme-Linked Immunosorbent Assay versus Chemiluminescent Immunoassay: A General Overview

Published:2023-02-08 Issue: Volume:18 Page:1
ISSN:2765-8910
Container-title:Global Journal of Medical, Pharmaceutical, and Biomedical Update
language:en
Short-container-title:GJMPBU

Author:

Khan Muddasir¹,Shah Syed Hussain²,Salman Muhammad²,Abdullah Mr²,Hayat Fawad¹,Akbar Sajeela¹

Affiliation:

1. Centre of Biotechnology and Microbiology, University of Peshawar, Peshawar, Pakistan

2. Department of Health and Biological Sciences, Abasyn University Peshawar, Peshawar, Pakistan,

Abstract

Enzyme-linked immunosorbent assay (ELISA) technique measures antigens, antibodies, and protein reactions in biological samples by enzymatic reactions. The chemiluminescence immunoassay (CLIA) technique determines sample concentrations based on the intensity of the light emitted by a chemical and biological reaction. This review provides an overview to understand the ELSIA and CLIA methods with their types and comparison. ELISA and CLIA methods were compared based on previous literature studies. In conclusion, CLIA is found highly sensitive, specific, and rapid, as compared to ELISA, but CLIA is an expensive method as compared to ELISA.

Publisher

Scientific Scholar

Subject

General Medicine

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