Analysis of bacteriophage deoxyribonucleic acid sequences methylated by host- and R-factor-controlled enzymes

Author:

May M S,Hattman S

Abstract

Phages lambda and fd were propagated in Escherichia coli strains that have either host K-12 or the N-3 R-factor deoxyribonucleic acid-cytosine methylase activity. Pyrimidine tracts containing 3H-labeled 5-methylcytosine (MeC) were analyzed; in all cases, the major methylated sequence was 5' ... C-MeC-T ... 3'.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference14 articles.

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2. The isolation and properties of nonrestricting mutants of two different host specificities associated with drug resistance factors;Bannister D.;J. Gen. Microbiol.,1970

3. DNA substrate site for the EcoRII restriction endonuclease and modification methylase;Boyer H. W.;Nature (London) New Biol.,1973

4. The frequencies of certain sequences of nucleotides in deoxyribonucleic acid;Burton K.;Biochem. J.,1960

5. The enzymic methylation of ribonucleic acid and deoxyribonucleic acid. V. Purification and properties of the deoxyribonucleic acid-methylating activity of Escherichia coli;Gold M.;J. Biol. Chem.,1964

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