Abstract
The relationship between the production of hemagglutinin and sialidase activities by Clostridium perfringens was investigated by screening for mutants producing reduced levels of hemagglutinin activity. Twelve mutants were isolated; all produced reduced levels of sialidase activity and several had other altered phenotypic markers. Revertants that regained the ability to produce active hemagglutinin were isolated. All of these revertants produced increased sialidase activity. These results show that the production of hemagglutinin activity is directly related to the production of sialidase activity. Evidence is also presented that the processes of sporulation and the production of extracellular proteins are interrelated.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference36 articles.
1. Optimal conditions for mutagenesis by N-methyl-N'- nitro-N-nitrosoguanidine in E. coli K 12;Adelberg E.;Biochem. Biophys. Res. Commun.,1965
2. The sialic acids. VI. Purification and properties of sialidase from Clostridium perfringens;Cassidy J. T.;J. Biol. Chem.,1965
3. The nature and properties of the haemagglutinin of Cl. welchii;Collee J. G.;J. Pathol. Bacteriol.,1961
4. The relationship of the haemagglutinin of Cl. welchii to the neuraminidase and other soluble products of the organism;Collee J. G.;J. Pathol. Bacteriol.,1965
5. Cruickshank R. 1965. Medical microbiology 11th ed. p. 745-746. E. & S. Livingstone London.
Cited by
15 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献