L-Arabinose-ornithine-Irgasan medium for differentiating Serratia species

Author:

Gibson S,Friedman H

Abstract

A semisolid medium (designated Serratia differentiation medium) containing L-arabinose, ornithine, and selective inhibitor was used to differentiate three clinically encountered Serratia species. The inhibitor, Irgasan DP-300, was incorporated to eliminate false-positive reactions from most remaining Enterobacteriaceae. The suspected Serratia colony was inoculated as a stab into the medium. Serratia marcescens was indicated by a change in color from olive to purple following 18 h of incubation, whereas S. rubidaea (not listed in Bergey's Manual of Determinative Bacteriology) was indicated by a change to bright yellow. S. liquefaciens (described in Bergey's Manual of Determinative Bacteriology [8th ed., 1974] as Enterobacter liquefaciens) produced a small purple band at the top of the medium and a yellow or yellow-green butt. Absence of growth and color change following incubation indicates that the suspected colony is a non-Serratia. Thirty-six Serratia strains and 97 other Enterobacteriaceae and Pseudomonadaceae strains were tested. Two strains of the non-Serratia Enterobacteriaceae (one each of Citrobacter freundii and Proteus morganii) and two strains of Pseudomonas aeruginosa produced a color change in the medium. All of the Serratia strains tested were correctly identified using this medium, while 96% of the other species tested were inhibited.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference7 articles.

1. Ewing W. H. 1973. Differentiation of enterobacteriaceae by biochemical reactions (revised). Center for Disease Control Atlanta Ga.

2. Biochemical characterization of Serratia liquefaciens (Grimes and Hennerty) Bascomb et al. (formerly Enterobacter liquefaciens) and Serratia rubidaea (Stapp) comb. now. and designation of type and neotype strains;Ewing W. IL;Int. J. Syst. Bacteriol.,1973

3. Lennette E. H. E. H. Spaulding and J. P. Truant (ed.). 1974. Manual of clinical microbiology 2nd ed. American Society for Microbiology Washington D.C.

4. A selective medium for isolation of Serratia sp. from raw sewage;Lynch D. L.;Microbios Lett.,1976

5. Sakazaki R. 1974. Genus Serratia Bizio 1823 288 p. 326. In R. E. Buchanan and N. E. Gibbons (ed.) Bergey's manual of determinative bacteriology 8th ed. The Williams & Wilkins Co. Baltimore.

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