Novel Multiplex PCR Using Dual-Priming Oligonucleotides for Detection and Discrimination of the Mycobacterium tuberculosis Complex and M. bovis BCG-Reference-Cited by-同舟云学术

Novel Multiplex PCR Using Dual-Priming Oligonucleotides for Detection and Discrimination of the Mycobacterium tuberculosis Complex and M. bovis BCG

Published:2010-12 Issue:12 Volume:48 Page:4612-4614
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Lee Hye Ryun¹,Kim So Yeon¹,Chang Ho Eun²,Song Sang Hoon¹²,Lee Hye Seung³,Park Kyoung Un¹²,Song Junghan¹²,Kim Eui-Chong¹

Affiliation:

1. Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul

2. Department of Laboratory Medicine

3. Department of Pathology, Seoul National University Bundang Hospital, Seongnam, South Korea

Abstract

ABSTRACT We developed a novel multiplex PCR assay using dual-priming oligonucleotide primers targeting the RD1 gene for simultaneous identification of the Mycobacterium tuberculosis complex and M. bovis bacillus Calmette-Guérin (BCG). This assay would be useful both for detection of the M. tuberculosis complex and for differentiation of M. bovis BCG from pathogenic M. tuberculosis complex species.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00872-10

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