Antibody-Mediated Neutralization of Pertussis Toxin-Induced Mitogenicity of Human Peripheral Blood Mononuclear Cells-Reference-Cited by-同舟云学术

Antibody-Mediated Neutralization of Pertussis Toxin-Induced Mitogenicity of Human Peripheral Blood Mononuclear Cells

Published:2004-01 Issue:1 Volume:72 Page:615-620
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Millen Scott H.¹,Bernstein David I.²,Connelly Beverly²,Ward Joel I.³,Chang Swei-Ju³,Weiss Alison A.¹

Affiliation:

1. Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, Cincinnati, Ohio 45267

2. Division of Infectious Diseases, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio 45229

3. UCLA Center for Vaccine Research, Harbor-UCLA Medical Center, Torrance, California 90502

Abstract

ABSTRACT Antibody-mediated neutralization of pertussis toxin-induced proliferation of human peripheral blood mononuclear cells (PBMC) was assessed using alamarBlue and compared with results from the Chinese hamster ovary (CHO) cell assay using sera from vaccinated adults and convalescent children. Neutralization values for the CHO assay were similar for vaccinated and convalescent subjects; however. the convalescent group had higher titers in the PBMC assay. Results for pertussis toxin neutralization with the CHO assay appear to be distinct from those with the PBMC assay.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.72.1.615-620.2004

Reference39 articles.

1. Ahmed, S. A., R. M. Gogal, Jr., and J. E. Walsh. 1994. A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H]thymidine incorporation assay. J. Immunol. Methods170:211-224.

2. Bokoch, G. M., and A. G. Gilman. 1984. Inhibition of receptor-mediated release of arachidonic acid by pertussis toxin. Cell39:301-308.

3. Brandt, S. J., R. W. Dougherty, E. G. Lapetina, and J. E. Niedel. 1985. Pertussis toxin inhibits chemotactic peptide-stimulated generation of inositol phosphates and lysosomal enzyme secretion in human leukemic (HL-60) cells. Proc. Natl. Acad. Sci. USA82:3277-3280.

4. Cherry, J. D., J. Gornbein, U. Heininger, and K. Stehr. 1998. A search for serologic correlates of immunity to Bordetella pertussis cough illnesses. Vaccine16:1901-1906.

5. de Fries, R., and M. Mitsuhashi. 1995. Quantification of mitogen induced human lymphocyte proliferation: comparison of alamarBlue assay to 3H-thymidine incorporation assay. J. Clin. Lab. Anal.9:89-95.

Cited by 10 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Multiplexed bead-based assay for the simultaneous quantification of human serum IgG antibodies to tetanus, diphtheria, pertussis toxin, filamentous hemagglutinin, and pertactin;Frontiers in Immunology;2023-06-05

2. Evaluation of Anti-PT Antibody Response after Pertussis Vaccination and Infection: The Importance of Both Quantity and Quality;Toxins;2021-07-21

3. Bacterial Vaccine Antigen Discovery in the Reverse Vaccinology 2.0 Era: Progress and Challenges;Frontiers in Immunology;2018-10-08

4. Single Amino Acid Polymorphisms of Pertussis Toxin Subunit S2 (PtxB) Affect Protein Function;PLOS ONE;2015-09-16

5. Effect of glycounits on the antimicrobial properties and toxicity behavior of polymers based on quaternized DMAEMA;Biomacromolecules;2014-12-10