Transformation of Acinetobacter sp. Strain BD413(pFG4Δ nptII ) with Transgenic Plant DNA in Soil Microcosms and Effects of Kanamycin on Selection of Transformants-Reference-Cited by-同舟云学术

Transformation of Acinetobacter sp. Strain BD413(pFG4Δ nptII ) with Transgenic Plant DNA in Soil Microcosms and Effects of Kanamycin on Selection of Transformants

Published:2000-03 Issue:3 Volume:66 Page:1237-1242
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Nielsen Kaare M.¹,van Elsas Jan D.²,Smalla Kornelia³

Affiliation:

1. Unigen and Department of Botany, Norwegian University of Science and Technology, 7491 Trondheim, Norway1;

2. Research Institute for Plant Protection, IPO-DLO, 6700 GW Wageningen, The Netherlands2; and

3. Institute for Plant Virology, Microbiology and Biosafety, Federal Biological Research Centre for Agriculture and Forestry, (BBA), 38104 Braunschweig, Germany3

Abstract

ABSTRACT Here we show that horizontal transfer of DNA, extracted from transgenic sugar beets, to bacteria, based on homologous recombination, can occur in soil. Restoration of a 317-bp-deleted nptII gene in Acinetobacter sp. strain BD413(pFG4) cells incubated in sterile soil microcosms was detected after addition of nutrients and transgenic plant DNA encoding a functional nptII gene conferring bacterial kanamycin resistance. Selective effects of the addition of kanamycin on the population dynamics of Acinetobacter sp. cells in soil were found, and high concentrations of kanamycin reduced the CFU of Acinetobacter sp. cells from 10 9 CFU/g of soil to below detection. In contrast to a chromosomal nptII -encoded kanamycin resistance, the pFG4-generated resistance was found to be unstable over a 31-day incubation period in vitro.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.66.3.1237-1242.2000

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