Reductive Cleavage of Demeton- S -Methyl by Corynebacterium glutamicum in Cometabolism on More Readily Metabolizable Substrates

Author:

Girbal Laurence1,Hilaire Didier2,Leduc Sébastien1,Delery Laure1,Rols Jean-Luc1,Lindley Nicholas D.1

Affiliation:

1. Laboratoire de Biotechnologie-Bioprocédés, UMR CNRS 5504 and UMR INRA 792, Centre de Bioingénierie Gilbert Durand, INSA, 31077 Toulouse cedex 4,1 and

2. Centre d'Etudes du Bouchet, DGA, 91710 Vert-Le-Petit,2 France

Abstract

ABSTRACT Corynebacterium glutamicum is able to biotransform demeton- S -methyl, an organophosphorus compound, during cometabolism with more readily metabolizable substrates. Among the cosubstrates used, fructose is the growth substrate that is most favorable for demeton- S -methyl biotransformation. The reaction mechanism of demeton- S -methyl biotransformation involves reductive cleavage of an S-C bond, which leads to accumulation of dimethyl thiophosphate in the culture medium.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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