Affiliation:
1. Gut Microbiology and Immunology Division, Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, United Kingdom
2. Department of Medical Microbiology, University of Groningen, Groningen, The Netherlands
Abstract
ABSTRACT
Duplicate anaerobic fermentor systems were used to examine changes in a community of human fecal bacteria supplied with different carbohydrate energy sources. A panel of group-specific fluorescent in situ hybridization probes targeting 16S rRNA sequences revealed that the fermentors supported growth of a greater proportion of
Bacteroides
and a lower proportion of gram-positive anaerobes related to
Faecalibacterium prausnitzii
,
Ruminococcus flavefaciens-Ruminococcus bromii
,
Eubacterium rectale-Clostridium coccoides
, and
Eubacterium cylindroides
than the proportions in the starting fecal inoculum. Nevertheless, certain substrates, such as dahlia inulin, caused a pronounced increase in the number of bacteria related to
R. flavefaciens-R. bromii
and
E. cylindroides.
The ability of three strictly anaerobic, gram-positive bacteria to compete with the complete human fecal flora was tested in the same experiment by using selective plating to enumerate the introduced strains. The
Roseburia
-related strain A2-183
F
was able to grow on all substrates despite the fact that it was unable to utilize complex carbohydrates in pure culture, and it was assumed that this organism survived by cross-feeding. In contrast,
Roseburia intestinalis
L1-82
R
and
Eubacterium
sp. strain A2-194
R
survived less well despite the fact that they were able to utilize polysaccharides in pure culture, except that A2-194
R
was stimulated 100-fold by inulin. These results suggest that many low-G+C-content gram-positive obligate anaerobes may be selected against during in vitro incubation, although several groups were stimulated by inulin. Thus, considerable caution is necessary when workers attempt to predict the in vivo effects of probiotics and prebiotics from their effects in vitro.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
147 articles.
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