Affiliation:
1. Key Laboratory of Industrial Biotechnology of Ministry of Education, State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, Wuxi, China
Abstract
Free-living lactic acid bacteria often encounter acid stresses because of their organic acid-producing features. Several acid resistance mechanisms, such as the glutamate decarboxylase system, F
1
F
o
-ATPase proton pump, and alkali production, are usually employed to relieve growth inhibition caused by acids. The glutamate decarboxylase system is vital for GAD-containing lactic acid bacteria to protect cells from DNA damage, enzyme inactivation, and product yield loss in acidic habitats. In this study, we found that a MerR-type regulator, GlnR, was involved in glutamate-dependent acid resistance by directly regulating the transcription of the
gadR
gene and
gadCB
operon, resulting in an inhibition of GABA conversion from glutamate in
L. brevis
. This study represents a novel mechanism for GlnR's regulation of glutamate-dependent acid resistance and also provides a simple and novel strategy to engineer
Lactobacillus
strains to elevate their acid resistance as well as GABA conversion from glutamate.
Funder
National Natural Science Foundation of China
National Key R & D Program
Open Project Program for Key Laboratory of Industrial Biotechnology of Ministry of Education
National First-class Discipline Program of Light Industry Technology and Engineering
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
19 articles.
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