Whole-Genome Sequencing of Staphylococcus aureus Strain RN4220, a Key Laboratory Strain Used in Virulence Research, Identifies Mutations That Affect Not Only Virulence Factors but Also the Fitness of the Strain-Reference-Cited by-同舟云学术

Whole-Genome Sequencing of Staphylococcus aureus Strain RN4220, a Key Laboratory Strain Used in Virulence Research, Identifies Mutations That Affect Not Only Virulence Factors but Also the Fitness of the Strain

Published:2011-05 Issue:9 Volume:193 Page:2332-2335
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Nair Dhanalakshmi¹,Memmi Guido¹,Hernandez David²,Bard Jonathan³,Beaume Marie²,Gill Steven⁴,Francois Patrice²,Cheung Ambrose L.¹

Affiliation:

1. Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire 03755

2. Genomic Research Laboratory, Service of Infectious Diseases, University of Geneva Hospitals, Rue Gabrielle-Perret-Gentil, CH-1211, Geneva 14, Switzerland

3. New York State Center of Excellence for Bioinformatics and Life Sciences, 701 Ellicott Street, Buffalo, New York 14203

4. Department of Microbiology, University of Rochester, Rochester, New York 14620

Abstract

ABSTRACT Staphylococcus aureus RN4220, a cloning intermediate, is sometimes used in virulence, resistance, and metabolic studies. Using whole-genome sequencing, we showed that RN4220 differs from NCTC8325 and contains a number of genetic polymorphisms that affect both virulence and general fitness, implying a need for caution in using this strain for such studies.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00027-11

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