Structure-Based Stabilization of SOSIP Env Enhances Recombinant Ectodomain Durability and Yield-Reference-Cited by-同舟云学术

Structure-Based Stabilization of SOSIP Env Enhances Recombinant Ectodomain Durability and Yield

Published:2023-01-31 Issue:1 Volume:97 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Wrapp Daniel¹^ORCID,Mu Zekun¹²,Thakur Bhishem¹,Janowska Katarzyna¹,Ajayi Oluwatobi¹,Barr Maggie¹³,Parks Robert¹³,Mansouri Katayoun¹,Edwards Robert J.¹,Hahn Beatrice H.⁴⁵,Acharya Priyamvada¹⁶,Saunders Kevin O.¹²⁶⁷,Haynes Barton F.¹²³

Affiliation:

1. Duke Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina, USA

2. Department of Immunology, Duke University Medical Center, Durham, North Carolina, USA

3. Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA

4. Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA

5. Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA

6. Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA

7. Department of Microbiology and Molecular Genetics, Duke University Medical Center, Durham, North Carolina, USA

Abstract

Recent estimates have placed the number of new human immunodeficiency virus type 1 (HIV-1) infections at approximately 1.5 million per year, emphasizing the ongoing and urgent need for an effective vaccine. The envelope (Env) glycoprotein is the main focus of HIV-1 vaccine development, but, due to its inherent metastability, many Env variants are difficult to recombinantly express in the relatively large quantities that are required for biochemical studies and animal trials.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.01673-22

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