Chromosome-Encoded Broad-Spectrum Ambler Class A β-Lactamase RUB-1 from Serratia rubidaea-Reference-Cited by-同舟云学术

Chromosome-Encoded Broad-Spectrum Ambler Class A β-Lactamase RUB-1 from Serratia rubidaea

Published:2017-02 Issue:2 Volume:61 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Bonnin Rémy A.¹²³^ORCID,Didi Jennifer¹,Ergani Ayla¹,Lima Sandra¹,Naas Thierry¹²³⁴

Affiliation:

1. EA7361 “Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases,” Université Paris Sud, Université Paris Saclay, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicêtre, France

2. French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France

3. Joint Research Unit EERA “Evolution and Ecology of Resistance to Antibiotics,” Institut Pasteur-APHP-Université Paris Sud, Paris, France

4. Bactériologie-Hygiene Unit, APHP, Bicêtre Hospital, Le Kremlin-Bicêtre, France

Abstract

ABSTRACT Whole-genome sequencing of Serratia rubidaea CIP 103234 T revealed a chromosomally located Ambler class A β-lactamase gene. The gene was cloned, and the β-lactamase, RUB-1, was characterized. RUB-1 displayed 74% and 73% amino acid sequence identity with the GIL-1 and TEM-1 penicillinases, respectively, and its substrate profile was similar to that of the latter β-lactamases. Analysis by 5′ rapid amplification of cDNA ends revealed promoter sequences highly divergent from the Escherichia coli σ 70 consensus sequence. This work further illustrates the heterogeneity of β-lactamases among Serratia spp.

Funder

Université Paris SUd

Laboratory of Excellence LERMIT

Assistance Publique - Hôpitaux de Paris

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01908-16

Reference23 articles.

1. Serratia Infections: from Military Experiments to Current Practice