Determination of the Nucleotide Sequences of Heat Shock Operon groESL and the Citrate Synthase Gene ( gltA ) of Anaplasma ( Ehrlichia ) platys for Phylogenetic and Diagnostic Studies

Author:

Inokuma Hisashi123,Fujii Kaori123,Okuda Masaru123,Onishi Takafumi123,Beaufils Jean-Pierre123,Raoult Didier123,Brouqui Philippe123

Affiliation:

1. Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, 753-8515 Yamaguchi, Japan

2. Clinique Vétérinaire, route de Salinelles, 30250 Somieres

3. Unité des Rickettsies, Faculté de Médecine, Université de la Méditerranée, Marseille Cédex 5, France

Abstract

ABSTRACT The 1,670-bp nucleotide sequence of the heat shock operon groESL and the 1,236-bp sequence of the citrate synthase gene ( gltA ) of Anaplasma ( Ehrlichia ) platys were determined. The topology of the groEL - and gltA -based phylogenetic tree was similar to that derived from 16S rRNA gene analyses with distances. Both groESL - and gltA -based PCRs specific to A. platys were also developed based upon the alignment data.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

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