Usefulness of Electrophoretic Pattern of Cell Envelope Protein as a Taxonomic Tool for Fishhold Slime Moraxella Species

Author:

Chai Tuu-Jyi1

Affiliation:

1. Seafood Technology Division, Horn Point Environmental Laboratories, Center for Environmental & Estuarine Studies, University of Maryland, Cambridge, Maryland 21613

Abstract

Nine independent Moraxella cultures were isolated from the accumulated slime in fishholds of fishery trawlers. It is significant that none of these isolates was viable above 30°C, a temperature well below the usual incubation temperature for plate counts of food samples. The traditional taxonomic parameters showed no significant dissimilarities among these closely related marine organisms or between them and conventional moraxellas. However, cell envelope protein profiles examined on sodium dodecyl sulfate-polyacrylamide gels revealed that the organisms fell into several distinct groups. The cell envelope protein profile could be a simple and quick test to determine the fine relationships between individual isolates.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference14 articles.

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2. Buchanan R. E. and N. E. Gibbons (ed.). 1974. Bergey's manual of determinative bacteriology 8th ed. The Williams & Wilkins Co. Baltimore.

3. Escherichia coli K-12 tolF mutants: alterations in protein composition of the outer membrane;Chai T.;J. Bacteriol.,1977

4. Characteristics ofheavily mucoid bacterial isolated from fish pen slime;Chai T.;Appl. Microbiol.,1975

5. Outer membranes of gram-negative bacteria. XIX. Isolation from Pseudomonas aeruginosa PA01 and use in reconstitution and definition of the permeability barrier;Hancock R. F. W.;J. Bacteriol.,1978

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