Expression and Characterization of Flagella in Nonmotile Enteroinvasive Escherichia coli Isolated from Diarrhea Cases

Author:

Andrade Agda1,Girón Jorge A.2,Amhaz Juliana M. K.1,Trabulsi Luiz R.3,Martinez Marina B.1

Affiliation:

1. Departamento de Analises Clinicas e Toxicologicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo

2. Instituto de Ciencias, Benemérita Universidad Autonoma de Puebla, Puebla, Mexico

3. Laboratorio de Microbiologia, Instituto Butantan, São Paulo, Brazil

Abstract

ABSTRACT We report that enteroinvasive Escherichia coli (EIEC) serotypes considered to be nonmotile produce an unusually large (77 kDa) flagellin that is assembled into functional flagellum filaments that allow the bacteria to swim in modified motility agar. The EIEC flagellin showed N-terminal identity to most common enterobacterial flagellins, especially those of the E. coli H7 serotype. These data are important in terms of the epidemiology, evolution, and biology of EIEC.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

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