Effects of Neolamarckia cadamba leaves extract on microbial community and antibiotic resistance genes in cecal contents and feces of broilers challenged with lipopolysaccharides

Abstract

ABSTRACT The effects of Neolamarckia cadamba leaves extract (NCLE), with effective ingredients of flavonoids, on antibiotic resistance genes (ARGs) and relevant microorganisms in cecal contents and feces of broilers treated with or without lipopolysaccharide stimulation (LPS) were investigated. LPS stimulation increased ( P < 0.05) the relative abundance of ARGs and mobile genetic elements (MGEs), such as tet(W/N/W ), APH(3')-IIIa , ErmB , tet (44 ), ANT (6)-Ia , tet(O ), tet (32 ), Vang_ACT_CHL , myrA , ANT (6)-Ib , IncQ1 , tniB , and rep2 in cecal contents. However, the difference disappeared ( P > 0.05) when NCLE was added at the same time. These differential ARGs and MGEs were mainly correlated ( P < 0.01) with Clostridiales bacterium, Lachnospiraceae bacterium, and Candidatus Woodwardibium gallinarum . These species increased in LPS-stimulated broilers and decreased when NCLE was applied at the same time. In feces, LPS stimulation decreased ( P < 0.05) the relative abundance of tet(Q ), adeF , ErmF , Mef(En2 ), OXA-347 , tet (40 ), npmA , tmrB, CfxA3, and ISCrsp1, while the LPS + NCLE treated group showed no significant effect ( P > 0.05) on these ARGs. These differential ARGs and MGEs in feces were mainly correlated ( P < 0.01) with Clostridiales bacterium, Pseudoflavonifractor sp . An184, Flavonifractor sp . An10, Ruminococcaceae bacterium, etc. These species increased in LPS-stimulated broilers and increased when NCLE was applied at the same time. In conclusion, LPS stimulation and NCLE influenced microbial communities and associated ARGs in both cecal contents and feces of broilers. NCLE alleviated the change of ARGs and MGEs in LPS-induced broilers by maintaining the microbial balance. IMPORTANCE Antibiotics showed a positive effect on gut health regulation and growth performance improvement in livestock breeding, but the antimicrobial resistance threat and environment pollution problem are increasingly severe with antibiotics abuse. As alternatives, plant extract containing bioactive substances are increasingly used to improve immunity and promote productivity. However, little is known about their effects on diversity and abundance of ARGs. Here, we investigated the effects of NCLE, with effective ingredients of flavonoids, on ARGs and relevant microorganisms in cecal contents and feces of broilers treated with or without lipopolysaccharide stimulation. We found that NCLE reduced the abundance of ARGs in cecal contents of lipopolysaccharide-induced broilers by maintaining the microbial balance. This study provides a comprehensive view of cecal and fecal microbial community, ARGs, and MGEs of broiler following LPS stimulation and NCLE treatment. It might be used to understand and control ARGs dissemination in livestock production.