Phenotypic Recognition of Pyocyanine Mutants in Pseudomonas aeruginosa-Reference-Cited by-同舟云学术

Phenotypic Recognition of Pyocyanine Mutants in Pseudomonas aeruginosa

Published:1974-01 Issue:1 Volume:117 Page:312-314
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Carson Melinda¹,Jensen Roy A.¹

Affiliation:

1. Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77025

Abstract

Certain classes of pyocyanine mutants in Pseudomonas aeruginosa escape detection when screened in the presence of wild-type or other mutant cells. A technique is described for recognizing mutant phenotypes after cells are in individual agar wells. The procedure eliminates cross-feeding phenomena as well as the masking of mutant phenotypes by diffusing pyocyanine produced by nearby clones.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.117.1.312-314.1974

Reference4 articles.

1. Branch point metabolite for pyocyanine biosynthesis in Pseudomonas aeruginosa;Calhoun D. H.;J. Gen. Microbiol.,1972

2. Transductional analysis of methionine auxotrophs of Pseudomonas aeruginosa;Calhoun D. H.;J. Bacteriol.,1969

3. Two simple media for the demonstration of pyocyanine and fluorescein;King E. O.;J. Lab. Clin. Med.,1954

4. The branch point of pyocyanine biosynthesis;Longley R. P.;Can. J. Microbiol.,1972

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