Azo Dye Colony Staining Technique To Detect Fungal Mutants Lacking β-Glucosidase Activity-Reference-Cited by-同舟云学术

Azo Dye Colony Staining Technique To Detect Fungal Mutants Lacking β-Glucosidase Activity

Published:1983-01 Issue:1 Volume:45 Page:315-316
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Rissler Jane F.¹

Affiliation:

1. Department of Botany, University of Maryland, College Park, Maryland 20742

Abstract

A simple, rapid, convenient, easily interpreted azo dye colony-staining technique was developed to detect fungal mutants lacking β-glucosidase activity on a 6-bromo-2-naphthyl substrate. The procedure includes net replication of colonies and chemical restriction of colony growth to allow testing of many colonies per culture dish.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.45.1.315-316.1983

Reference14 articles.

1. Histochemical localization of 1B-glucosidases in roots of Zea mays. I. A simultaneous coupling azo-dye technique for the localization of ,Bglucosidase and ,B-galactosidase;Ashford A. E.;Protoplasma,1970

2. Neurospora. II. Methods of producing and detecting mutations concerned with nutritional requirements;Beadle G. W.;Am. J. Bot.,1945

3. Butler G. W. 1%5. The distribution of cyanoglucosides linamarin and lotaustralin in higher plants. Phytochemistry 4:127-131.

4. The colorimetric estimation and histochemical demonstration of 3-D-galactosidase;Cohen R. B.;J. Biol. Chem.,1952

5. Inhibition of glycosidases by aldonolactones of corresponding configuration;Conchie J.;Biochem. J.,1957

Cited by 6 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Detection of β-Glucosidase Activity in Polyacrylamide Gels with Esculin as Substrate;Applied and Environmental Microbiology;1994-12

2. Cellulase assay methods: a review;Journal of Biochemical and Biophysical Methods;1988-10

3. Screening of prokaryotes for cellulose- and hemicellulose-degrading enzymes;Methods in Enzymology;1988

4. Staining techniques for the detection of the individual components of cellulolytic enzyme systems;Methods in Enzymology;1988

5. 3 Enzyme Tests in Bacterial Identification;Methods in Microbiology;1988