Affiliation:
1. U.S. Army Biological Laboratories, Fort Detrick, Frederick, Maryland
Abstract
Puziss, Milton
(Fort Detrick, Frederick, Md.) and
George G. Wright
. Studies on immunity in anthrax. X. Gel-adsorbed protective antigen for immunization of man. J. Bacteriol.
85:
230–236. 1963.—Protective antigen in filtrates of anaerobic cultures of
Bacillus anthracis
was not readily precipitable by alum or other aluminum salts, but was adsorbed onto a preformed aluminum hydroxide gel under specific conditions. The adsorbed product was highly effective in immunizing rabbits, guinea pigs, and mice against challenge with virulent
B. anthracis
spores. Gel-adsorbed anaerobic antigens preserved with 1:10,000 thimerosal were unstable on storage at 4 C; replacement of this preservative with 1:40,000 benzethonium chloride produced a more stable product. Addition of 0.0009% formalin further increased the stability during accelerated aging at 37 C. The final product had high protective activity in animals and was well tolerated in man.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference18 articles.
1. Rapid lethal effect in rats of a third component found upon fractionating the toxin of Bacillus anthracis;BEALL F. A.;J. Bacteriol.,1962
2. Field evaluation of a human anthrax vaccine;BRACHMAN P. S.;Am. J. Public Health,1962
3. CARDELLA M. A. M. A. FIOCK AND G. G. WRIGHT. 1958. Immunologic response of animals to purified pentavalent ABCDE botulinum toxoid. Bacteriol. Proc. p. 78.
4. The preservation of poliomyelitis vaccine with stabilized Merthiolate;DAVISSON E.;J. Lab. Clin. Med.,1956
5. Protamine insulinate;HAGEDORN H. C.;J. Am. Med. Assoc.,1936
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