Affiliation:
1. Department of Biological Sciences, University of California, Santa Barbara 93106.
Abstract
The centromere locus from linkage group VII of Neurospora crassa has been cloned, characterized, and physically mapped. The centromeric DNA is contained within a 450-kb region that is recombination deficient, A+T-rich, and contains repetitive sequences. Repetitive sequences from within this region hybridize to a family of repeats located at or near centromeres in all seven linkage groups of N. crassa. Genomic Southern blots and sequence analysis of these repeats revealed a unique centromere structure containing a divergent family of centromere-specific repeats. The predominantly transitional differences between copies of the centromere-specific sequence repeats and their high A+T content suggest that their divergence was mediated by repeat-induced point (RIP) mutations.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Reference46 articles.
1. Evidence for transcriptional regulation of orotidine-5'-phosphate decarboxylase in yeast by hybridization of mRNA to the yeast structural gene cloned in Escherichia coli;Bach M. L.;Proc. Natl. Acad. Sci. USA,1979
2. Purification of the yeast centromere binding protein CP1 and a mutational analysis of its binding site;Baker R. E.;J. Biol. Chem.,1989
3. Functional analysis of a centromere from fission yeast: a role for centromere-specific repeated DNA sequences;Baum M.;Mol. Cell. Biol.,1990
4. Isolation of a Saccharomyces cerevisiae centromere DNA-binding protein, its human homolog, and its possible role as a transcription factor;Bram R. J.;Mol. Cell. Biol.,1987
5. Brinkley B. R. M. M. Valdivia A. Tousson and R. D. Balezon. 1989. The kinetochore: structure and molecular organization p. 77-118. In J. S. Hyams and B. R. Brinkley (ed.) Mitosis: molecules and mechanisms. Academic Press London.
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献