Detection of Occult Scedosporium Species in Respiratory Tract Specimens from Patients with Cystic Fibrosis by Use of Selective Media

Author:

Blyth C. C.12,Harun A.2,Middleton P. G.34,Sleiman S.1,Lee O.1,Sorrell T. C.14,Meyer W.24,Chen S. C. A.124

Affiliation:

1. Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR, Westmead Hospital, Westmead, Sydney, NSW 2145, Australia

2. Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Westmead Hospital

3. Department of Respiratory Medicine, Westmead Hospital

4. Sydney Medical School—Western, University of Sydney, Sydney, Australia

Abstract

ABSTRACT Respiratory samples from cystic fibrosis outpatients were cultured on Sabouraud's dextrose agar (SABD) containing antibiotics, Mycosel, and Scedosporium -selective medium (SceSel+). Thirty-two (14.7%) of 218 specimens from 11/69 (15.9%) patients yielded a Scedosporium sp., most frequently Scedosporium aurantiacum (17/218). Scedosporium was recovered on SceSel+, Mycosel, and SABD from 90.6%, 50.0%, and 46.9% of the specimens tested, respectively.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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