Execution times of macromolecular synthetic processes involved in the induction of allophanate hydrolase at 15 degrees C

Abstract

We have observed that transcription, involved in production of allophanate hydrolase, is completed 2.5 min after the addition of inducer at 15 degrees C. The rna1 gene product must be functional up unti 10 min; protein synthesis is initiated at 20 min and is terminated by 24 min. Two minutes later, active enzyme appears. The results confirm our earlier observations and eliminate any uncertainty that might have clouded identification of the time within the lag period that is occupied by ribonucleic acid synthesis.