Isolation and Characterization of an Escherichia coli K-12 Mutant with a Temperature-Sensitive RecA − Phenotype

Abstract

A mutation that causes a temperature-sensitive RecA − phenotype was identified in a derivative of a PolA − strain that failed to grow at high temperature. The mutant allele ( recA200 ) was shown to be linked to cysC , conferred a sharply temperature-sensitive, ultraviolet-sensitive Rec − phenotype in the range 35 to 42 C, and in crosses failed to show complementation at 42 C with Hfr's that transferred recA − . Double mutants that carried both recA200 and polA were examined for ability to grow and synthesize DNA at restrictive temperatures.