Affiliation:
1. Department of Genetics, Albert Einstein Medical Center, Philadelpia, Pennsylvania 19141
Abstract
Loss of photoreversibility (LOP) of the ultraviolet (UV) damage which prevents reinitiation of deoxyribonucleic acid (DNA) replication occurred with incubation of
Escherichia coli
B/r
thy trp
cultures after UV doses of 240, 320, and 400 ergs/mm
2
. LOP occurred at the time of reinitiation of DNA replication in the cultures (i.e., after postirradiation lag periods of 45 min or more). Neither the absence of thymine nor the absence of tryptophan prevented LOP of the damage to DNA replication, suggesting that neither DNA replication nor protein synthesis is necessary for the process. These findings suggest that attempted initiation of DNA replication results in transformation of pyrimidine damage into permanent damage to chromosome structure at the reinitiation site.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference15 articles.
1. Replication of the bacterial chromosome: location of new initiation sites after irradiation;Billen D.;J. Bacteriol.,1969
2. Macromolecular synthesis in bacterial recovery from ultraviolet light;Doudney C.;Nature (London),1959
3. Doudney C. 0. 1965. Ultraviolet light effects on deoxyribonucleic acid replication p. 120-141. In Cellular radiation biology Williams and Wilkins Co. Baltimore.
4. Ultraviolet light effects on the bacterial cell;Doudney C.;Curr. Top. Microbiol. Immunol.,1968
5. Increased resistance of DNA replication to ultraviolet light damage in amino-acid starved bacteria;Doudney C.;Biochim. Biophys. Acta,1971
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