Molecular Detection of Mycobacterium tuberculosis from Stools in Young Children by Use of a Novel Centrifugation-Free Processing Method-Reference-Cited by-同舟云学术

Molecular Detection of Mycobacterium tuberculosis from Stools in Young Children by Use of a Novel Centrifugation-Free Processing Method

Published:2018-09 Issue:9 Volume:56 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Walters Elisabetta¹,Scott Lesley²,Nabeta Pamela³,Demers Anne-Marie¹,Reubenson Gary⁴,Bosch Corné¹,David Anura²,van der Zalm Marieke¹,Havumaki Joshua³⁵,Palmer Megan¹,Hesseling Anneke C.¹,Ncayiyana Jabulani⁶⁷,Stevens Wendy²⁸,Alland David⁹,Denkinger Claudia³,Banada Padmapriya⁹^ORCID

Affiliation:

1. Desmond Tutu TB Centre, Stellenbosch University, Paediatrics and Child Health, Faculty of Medicine and Health Sciences, Cape Town, South Africa

2. Molecular Medicine and Haematology, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa

3. Foundation for Innovative New Diagnostics, Geneva, Switzerland

4. Rahima Moosa Mother & Child Hospital, University of the Witwatersrand, Faculty of Health Sciences, Paediatrics and Child Health, Johannesburg, South Africa

5. Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, Michigan, USA

6. Division of Epidemiology & Biostatistics, School of Public Health and Family Medicine, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa

7. Division of Epidemiology & Biostatistics, School of Public Health, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa

8. National Priority Program of the National Health Laboratory Service, Johannesburg, South Africa

9. Rutgers, New Jersey Medical School, Faculty of Medicine, Newark, New Jersey, USA

Abstract

The microbiological diagnosis of tuberculosis (TB) in children is challenging, as it relies on the collection of relatively invasive specimens by trained health care workers, which is not feasible in many settings. Mycobacterium tuberculosis is detectable from the stools of children using molecular methods, but processing stool specimens is resource intensive.

Funder

Foundation for Innovative New Diagnostics

Tuberculosis Trials Consortium

IMPAACT network

South African Medical Research Council

Bill and Melinda Gates Foundation

Australian Agency for International Development

South African National Research Foundation

Publisher

American Society for Microbiology

Subject

Microbiology (medical)