Development and Validation of a PCR Assay To Detect the Prairie Epidemic Strain of Pseudomonas aeruginosa from Patients with Cystic Fibrosis-Reference-Cited by-同舟云学术

Development and Validation of a PCR Assay To Detect the Prairie Epidemic Strain of Pseudomonas aeruginosa from Patients with Cystic Fibrosis

Published:2016-02 Issue:2 Volume:54 Page:489-491
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Workentine M.¹,Poonja A.²,Waddell B.²,Duong J.³,Storey D. G.²³,Gregson D.²⁴,Somayaji R.²⁴,Rabin H. R.²⁴,Surette M. G.²⁵⁶⁷,Parkins M. D.²⁴

Affiliation:

1. Department of Veterinary Medicine, University of Calgary, Calgary, Canada

2. Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Canada

3. Department of Biological Sciences, University of Calgary, Calgary, Canada

4. Department of Medicine, University of Calgary, Calgary, Canada

5. Department of Medicine, McMaster University, Hamilton, Ontario, Canada

6. Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada

7. The Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, Ontario, Canada

Abstract

ABSTRACT The monitoring of epidemic Pseudomonas aeruginosa is important for cystic fibrosis (CF) infection control. The prairie epidemic strain (PES) is common in western Canadian CF clinics. Using whole-genome sequencing, we identified a novel genomic island and developed a PCR assay for PES. Against a collection of 186 P. aeruginosa isolates, the assay had 98% sensitivity and 100% specificity.

Funder

Cystic Fibrosis Canada

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02603-15

Reference23 articles.

1. Diagnostic multiplex PCR assay for the identification of the Liverpool, Midlands 1 and Manchester CF epidemic strains of Pseudomonas aeruginosa

2. A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa

3. Use of Subtractive Hybridization To Identify a Diagnostic Probe for a Cystic Fibrosis Epidemic Strain of Pseudomonas aeruginosa