A Molecular Biology Tool Kit for the Phototrophic Firmicute Heliobacterium modesticaldum-Reference-Cited by-同舟云学术

A Molecular Biology Tool Kit for the Phototrophic Firmicute Heliobacterium modesticaldum

Published:2019-10 Issue:19 Volume:85 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Baker Patricia L.¹²,Orf Gregory S.¹²^ORCID,Khan Zahid¹,Espinoza Levi¹,Leung Sabrina¹,Kevershan Kimberly¹,Redding Kevin E.¹²^ORCID

Affiliation:

1. School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA

2. Center for Bioenergy and Photosynthesis, Arizona State University, Tempe, Arizona, USA

Abstract

Heliobacteria are anoxygenic phototrophic bacteria with the simplest known photosynthetic apparatus. They are unique in using bacteriochlorophyll g as their main pigment and lacking a peripheral antenna system. Until now, research on this organism has been hampered by the lack of a genetic transformation system. Without such a system, gene knockouts, site-directed mutations, and gene expression studies cannot be performed to help us further understand or manipulate the organism. Here we report the genetic transformation of a heliobacterium, which should enable future genetic studies in this unique phototrophic organism.

Funder

U.S. Department of Energy

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01287-19

Reference33 articles.

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