Affiliation:
1. Department of Microbiology, University of Georgia, Athens, Georgia 30602
Abstract
Transpositional mutagenesis of
Thiobacillus novellus
by Tn
501
was achieved by means of the incompatibility of IncP plasmids. Tn
501
insertion caused three types of mutant phenotypes: isoleucine auxotrophy, lysine auxotrophy, and a reduced ability to oxidize reduced sulfur compounds and to fix CO
2
. Oxidation rates for elemental sulfur (S
0
), thiosulfate (S
2
O
3
2−
), and tetrathionate (S
4
O
6
2−
) in mutants of the latter type were reduced relative to those of the nonmutant control strain. Incorporation of labeled bicarbonate (H
14
CO
3
−
) was also significantly impaired. Although suicide vehicles were not useful for the introduction of transposons into
T. novellus
, this method was effective for the Tn
1721
-induced mutagenesis of
Thiobacillus versutus.
Tn
1721
insertions resulted in the loss of the natural resistance of
T. versutus
to arsenate and gentamicin and in auxotrophies for isoleucine-valine, arginine, phenylalanine, valine, and panthothenate. Transpositional mutagenesis by either method should prove to be a useful tool for further study of these and other members of the genus
Thiobacillus.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
13 articles.
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