High Production of Thermostable β-Galactosidase of Bacillus stearothermophilus in Bacillus subtilis-Reference-Cited by-同舟云学术

High Production of Thermostable β-Galactosidase of Bacillus stearothermophilus in Bacillus subtilis

Published:1985-06 Issue:6 Volume:49 Page:1547-1549
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Hirata Haruhisa¹,Negoro Seiji¹,Okada Hirosuke¹

Affiliation:

1. Department of Fermentation Technology, Osaka University, Yamada-oka, Suita-shi, Osaka 565, and Wakamoto Pharmaceutical Co., Ltd., Nihonbashimuromachi 1-8, Chuo-ku, Tokyo 103, 2 Japan

Abstract

By cloning the β-galactosidase gene of Bacillus stearothermophilus IAM11001 (ATCC 8005) into Bacillus subtilis , enzyme production was enhanced 50 times. β-Galactosidase could be purified to 80% homogeneity by incubating the cell extract of B. subtilis at 70°C for 15 min, followed by centrifugation to remove the denatured proteins. Because of its heat stability and ease of production, β-galactosidase is suitable for application in industrial processes.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.49.6.1547-1549.1985

Reference8 articles.

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