Metagenomic Next-Generation Sequencing of the 2014 Ebola Virus Disease Outbreak in the Democratic Republic of the Congo-Reference-Cited by-同舟云学术

Metagenomic Next-Generation Sequencing of the 2014 Ebola Virus Disease Outbreak in the Democratic Republic of the Congo

Published:2019-09 Issue:9 Volume:57 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Li Tony¹²,Mbala-Kingebeni Placide³,Naccache Samia N.¹²,Thézé Julien⁴,Bouquet Jerome¹²,Federman Scot¹²,Somasekar Sneha¹²,Yu Guixia¹²,Sanchez-San Martin Claudia¹²,Achari Asmeeta¹²,Schneider Bradley S.⁵,Rimoin Anne W.⁶,Rambaut Andrew⁷,Nsio Justus⁸,Mulembakani Prime³,Ahuka-Mundeke Steve³,Kapetshi Jimmy³,Pybus Oliver G.⁴,Muyembe-Tamfum Jean-Jacques³,Chiu Charles Y.¹²⁹^ORCID

Affiliation:

1. Department of Laboratory Medicine, University of California, San Francisco, San Francisco, California, USA

2. UCSF-Abbott Viral Diagnostics and Discovery Center, University of California, San Francisco, San Francisco, California, USA

3. Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of the Congo

4. Department of Zoology, University of Oxford, Oxford, United Kingdom

5. Etiologic, Inc., San Francisco, California, USA

6. Department of Epidemiology, School of Public Health, University of California, Los Angeles, Los Angeles, California, USA

7. Institute of Evolutionary Biology, University of Edinburgh, Edinburgh, United Kingdom

8. Ministry of Public Health, Kinshasa, Democratic Republic of the Congo

9. Department of Medicine, Division of Infectious Diseases, University of California, San Francisco, San Francisco, California, USA

Abstract

We applied metagenomic next-generation sequencing (mNGS) to detect Zaire Ebola virus (EBOV) and other potential pathogens from whole-blood samples from 70 patients with suspected Ebola hemorrhagic fever during a 2014 outbreak in Boende, Democratic Republic of the Congo (DRC) and correlated these findings with clinical symptoms. Twenty of 31 patients (64.5%) tested in Kinshasa, DRC, were EBOV positive by quantitative reverse transcriptase PCR (qRT-PCR).

Funder

Abbott Laboratories, Inc.

National Institutes of Health

European Research Council

Wellcome Trust core award

Oxford Martin School

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00827-19

Reference38 articles.

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4. Viral hemorrhagic fever in the tropics: Report from the task force on tropical diseases by the World Federation of Societies of Intensive and Critical Care Medicine

5. CDC. 2018. Case counts. The 2014–2016 outbreak in West Africa has ended. https://www.cdc.gov/vhf/ebola/history/2014-2016-outbreak/case-counts.html. Accessed 25 May 2018.

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