Two Accessory Proteins Govern MmpL3 Mycolic Acid Transport in Mycobacteria

Author:

Fay Allison1,Czudnochowski Nadine23,Rock Jeremy M.4,Johnson Jeffrey R.567,Krogan Nevan J.567,Rosenberg Oren238,Glickman Michael S.1910ORCID

Affiliation:

1. Immunology Program, Sloan Kettering Institute, New York, New York, USA

2. Program for Microbial Pathogenesis, University of California, San Francisco, San Francisco, California, USA

3. Division of Infectious Diseases, Department of Medicine, University of California, San Francisco, San Francisco, California, USA

4. Laboratory of Host-Pathogen Biology, The Rockefeller University, New York, New York, USA

5. Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California, USA

6. Quantitative Biosciences Institute (QBI), University of California, San Francisco, San Francisco, California, USA

7. The J. David Gladstone Institutes, San Francisco, California, USA

8. Chan-Zuckerberg Biohub, San Francisco, California, USA

9. Division of Infectious Diseases, Memorial Sloan Kettering Cancer Center, New York, New York, USA

10. Immunology and Microbial Pathogenesis Graduate Program, Weill Cornell Graduate School, New York, New York, USA

Abstract

The cell envelope of Mycobacterium tuberculosis , the bacterium that causes the disease tuberculosis, is a complex structure composed of abundant lipids and glycolipids, including the signature lipid of these bacteria, mycolic acids. In this study, we identified two new components of the transport machinery that constructs this complex cell wall. These two accessory proteins are in a complex with the MmpL3 transporter. One of these proteins, TtfA, is required for mycolic acid transport and cell viability, whereas the other stabilizes the MmpL3 complex. These studies identify two new components of the essential cell envelope biosynthetic machinery in mycobacteria.

Funder

HHS | NIH | National Cancer Institute

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

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