Isolation and Culture of Single Microbial Cells by Laser Ejection Sorting Technology-Reference-Cited by-同舟云学术

Isolation and Culture of Single Microbial Cells by Laser Ejection Sorting Technology

Published:2022-02-08 Issue:3 Volume:88 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Liang Peng¹²,Liu Bo¹²,Wang Yun³,Liu Kunxiang¹²,Zhao Yinping⁴,Huang Wei E.³⁵^ORCID,Li Bei¹

Affiliation:

1. State Key laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun, People’s Republic of China

2. University of Chinese Academy of Sciences, Beijing, People’s Republic of China

3. Oxford Suzhou Centre for Advanced Research (OSCAR), University of Oxford, Jiangsu, People’s Republic of China

4. Academy for engineering and technology, Fudan University, Shanghai, People’s Republic of China

5. Department of Engineering Science, University of Oxford, Oxford, United Kingdom

Abstract

We developed a laser-induced forward transfer (LIFT) technology to accurately isolate single live microbial cells. The cultivation recovery rates of the ejected single cells were 63% for Saccharomyces cerevisiae , 22% for E. coli DH5α, and 74% for Lactobacillus rhamnosus GG (LGG).

Funder

NERC Environmental Bioinformatics Centre

UKRI | Engineering and Physical Sciences Research Council

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.01165-21

Reference44 articles.

1. Single-Cell Ecophysiology of Microbes as Revealed by Raman Microspectroscopy or Secondary Ion Mass Spectrometry Imaging

2. Single cell stable isotope probing in microbiology using Raman microspectroscopy