Evaluation of a Prototype Real-Time PCR Assay for Carcinogenic Human Papillomavirus (HPV) Detection and Simultaneous HPV Genotype 16 (HPV16) and HPV18 Genotyping-Reference-Cited by-同舟云学术

Evaluation of a Prototype Real-Time PCR Assay for Carcinogenic Human Papillomavirus (HPV) Detection and Simultaneous HPV Genotype 16 (HPV16) and HPV18 Genotyping

Published:2009-10 Issue:10 Volume:47 Page:3344-3347
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Castle Philip E.¹,Sadorra Mark²,Lau Tiffany²,Aldrich Carrie²,Garcia Francisco A. R.³,Kornegay Janet²

Affiliation:

1. Division of Cancer Epidemiology, National Cancer Institute, NIH, DHHS, Bethesda, Maryland

2. Roche Molecular Systems, Alameda, California

3. University of Arizona Health Sciences Center, Tucson, Arizona

Abstract

ABSTRACT Results from a prototype real-time PCR assay that separately detected human papillomavirus genotype 16 (HPV16), HPV18, and 12 other carcinogenic HPV genotypes in aggregate (cobas 4800 HPV test) and results from a PCR assay that detects 37 HPV genotypes individually (Linear Array) were compared using a convenience sample of cervical specimens ( n = 531). The percentage of total agreement between the two assays was 94.7% (95% confidence interval, 92.5 to 96.5%). The Linear Array test was more likely than cobas 4800 HPV test to test positive for the 12 other carcinogenic HPV genotypes among women without evidence of cervical disease ( P = 0.004).

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00725-09

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