Controlled Proteolysis of an Essential Virulence Determinant Dictates Infectivity of Lyme Disease Pathogens-Reference-Cited by-同舟云学术

Controlled Proteolysis of an Essential Virulence Determinant Dictates Infectivity of Lyme Disease Pathogens

Published:2022-05-19 Issue:5 Volume:90 Page:
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Thakur Meghna¹,Bista Sandhya¹,Foor Shelby D.¹,Dutta Shraboni¹,Yang Xiuli¹,Ronzetti Michael¹²,Rana Vipin S.¹,Kitsou Chrysoula¹,Linden Sara B.³,Altieri Amanda S.³,Baljinnyam Bolormaa²,Nelson Daniel C.¹³⁴^ORCID,Simeonov Anton²,Pal Utpal¹⁴^ORCID

Affiliation:

1. Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA

2. National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland, USA

3. Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland, USA

4. Virginia-Maryland College of Veterinary Medicine, College Park, Maryland, USA

Abstract

The Borrelia burgdorferi BB0323 protein undergoes a complex yet poorly defined proteolytic maturation event that generates N-terminal and C-terminal proteins with essential functions in cell growth and infection. Here, we report that a borrelial protease, B. burgdorferi high temperature requirement A protease (BbHtrA), cleaves BB0323 between asparagine (N) and leucine (L) at positions 236 and 237, while the replacement of these residues with alanine in the mutant protein prevents its cleavage, despite preserving its normal secondary structure.

Funder

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/iai.00059-22

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