A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot ( Scophthalmus maximus )-Reference-Cited by-同舟云学术

A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot ( Scophthalmus maximus )

Published:2007-03-15 Issue:6 Volume:189 Page:2575-2579
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Sun Boguang¹,Zhang Xiao-Hua¹,Tang Xuexi¹,Wang Shushan¹,Zhong Yingbin¹,Chen Jixiang¹,Austin Brian²

Affiliation:

1. Department of Marine Biology, Ocean University of China, Qingdao, China

2. School of Life Sciences, Heriot-Watt University, Edinburgh EH14 4AS, United Kingdom

Abstract

ABSTRACT Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity and for its virulence in fish.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.01650-06

Reference26 articles.

1. Akoh, C. C., G.-C. Lee, Y.-C. Liaw, T.-H. Huang, and J.-F. Shaw. 2004. GDSL family of serine esterases/lipases. Prog. Lipid Res. 43 : 534-552.

2. Austin B. and D. A. Austin. 1989. Methods for the microbiological examination of fish and shellfish. Ellis Horwood Chichester United Kingdom.

3. Austin B. and D. A. Austin. 1999. Bacterial pathogens: disease of farmed and wild fish. Springer-Praxis Godalming United Kingdom.

4. Austin, B., and X.-H. Zhang. 2006. Vibrio harveyi: a significant pathogen of marine vertebrates and invertebrates. Lett. Appl. Microbiol. 43 : 119-124.

5. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

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