A Laboratory Medicine Best Practices Systematic Review and Meta-analysis of Nucleic Acid Amplification Tests (NAATs) and Algorithms Including NAATs for the Diagnosis of Clostridioides ( Clostridium ) difficile in Adults

Author:

Kraft Colleen S.1ORCID,Parrott J. Scott23,Cornish Nancy E.4,Rubinstein Matthew L.4,Weissfeld Alice S.5,McNult Peggy6,Nachamkin Irving7,Humphries Romney M.8,Kirn Thomas J.23,Dien Bard Jennifer910,Lutgring Joseph D.1,Gullett Jonathan C.11,Bittencourt Cassiana E.12,Benson Susan1314ORCID,Bobenchik April M.15,Sautter Robert L.16,Baselski Vickie17,Atlas Michel C.18,Marlowe Elizabeth M.19,Miller Nancy S.2021,Fischer Monika22,Richter Sandra S.23,Gilligan Peter24,Snyder James W.18

Affiliation:

1. Emory University School of Medicine, Atlanta, Georgia, USA

2. Department of Interdisciplinary Studies, School of Health Professions, Rutgers University, Newark, New Jersey, USA

3. Department of Epidemiology, School of Public Health, Rutgers University, Piscataway, New Jersey, USA

4. Centers for Disease Control and Prevention, Atlanta, Georgia, USA

5. Microbiology Specialists Incorporated, Houston, Texas, USA

6. American Society for Microbiology, Washington, DC, USA

7. Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA

8. Accelerate Diagnostics, Tucson, Arizona, USA

9. Children’s Hospital Los Angeles, Los Angeles, California, USA

10. Keck School of Medicine, University of Southern California, Los Angeles, California, USA

11. Kaiser Permanente (Southern California Permanente Medical Group) Regional Reference Laboratories, Greater Los Angeles, Los Angeles, California, USA

12. University of California—Irvine, Orange, California, USA

13. PathWest Laboratory Medicine, Perth, Western Australia, Australia

14. University of Western Australia, Perth, Western Australia, Australia

15. Rhode Island Hospital/Lifespan Academic Medical Center, Warren Alpert Medical School of Brown University, Providence, Rhode Island, USA

16. RL Sautter Consulting LLC, Lancaster, South Carolina, USA

17. University of Tennessee Health Science Center, Memphis, Tennessee, USA

18. Kornhauser Health Sciences Library, University of Louisville, Louisville, Kentucky, USA

19. Roche Molecular Systems, Pleasanton, California, USA

20. Boston Medical Center, Boston, Massachusetts, USA

21. Boston University School of Medicine, Boston, Massachusetts, USA

22. Indiana University, Indianapolis, Indiana, USA

23. Cleveland Clinic, Cleveland, Ohio, USA

24. University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA

Abstract

The evidence base for the optimal laboratory diagnosis of Clostridioides ( Clostridium ) difficile in adults is currently unresolved due to the uncertain performance characteristics and various combinations of tests. This systematic review evaluates the diagnostic accuracy of laboratory testing algorithms that include nucleic acid amplification tests (NAATs) to detect the presence of C. difficile .

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Microbiology (medical),Public Health, Environmental and Occupational Health,General Immunology and Microbiology,Epidemiology

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