Cloning and expression in Escherichia coli of chromosomal mercury resistance genes from a Bacillus sp-Reference-Cited by-同舟云学术

Cloning and expression in Escherichia coli of chromosomal mercury resistance genes from a Bacillus sp

Published:1987-10 Issue:10 Volume:169 Page:4848-4851
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Wang Y¹,Mahler I¹,Levinson H S¹,Halvorson H O¹

Affiliation:

1. Rosenstiel Basic Medical Sciences Research Center, Waltham, Massachusetts.

Abstract

A 7.9-kilobase (kb) chromosomal fragment was cloned from a mercury-resistant Bacillus sp. In Escherichia coli, in the presence of a second plasmid carrying functional transport genes, resistance to HgCl2 and to phenylmercury acetate (PMA) was expressed. Shortening the cloned fragment to 3.8 kb abolished resistance to PMA but not to HgCl2. In Bacillus subtilis, the 3.8-kb fragment produced mercuric reductase constitutively but did not produce resistance to HgCl2 or to PMA.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.169.10.4848-4851.1987

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