Affiliation:
1. Department of Applied Biological Science, Science University of Tokyo, Chiba, Japan.
Abstract
When cells competent for genetic transformation of Streptococcus pneumoniae which could bind and enable entry of extracellular DNA molecules were treated with LiCl, they released a nickase that introduced nicks into a double-stranded DNA in the presence of EDTA. The nickase was specific for competent cells and coupled with DNA-binding activity. Furthermore, when noncompetent cells were treated with LiCl, they released the putative receptors for the competence activator.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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