Propagation and Purification of High-Titer Human Cytomegalovirus-Reference-Cited by-同舟云学术

Propagation and Purification of High-Titer Human Cytomegalovirus

Published:1971-11 Issue:5 Volume:22 Page:914-918
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Chambers R. W.¹²³⁴,Rose J. A.¹²³⁴,Rabson A. S.¹²³⁴,Bond H. E.¹²³⁴,Hall W. T.¹²³⁴

Affiliation:

1. Department of Pathology, Georgetown University School of Medicine, Washington, D.C. 20007

2. Laboratory of Biology of Viruses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

3. Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

4. Electro-Nucleonics Laboratories, Inc., Bethesda, Maryland

Abstract

High-titered yields of human cytomegalovirus (CMV), strain AD 169, were produced in WI-38 cells in large roller bottles. Maximum plaque titers were observed by the 4th day after infection at which time infectivity in the medium was 200 times greater than that associated with the cells. Virus released into the medium was recovered by sedimentation in a sucrose gradient in a continuous-flow centrifuge rotor. Maximal viral infectivity was found at a sucrose concentration of 42%, equivalent to a density of 1.18 g/cm 3 . Deoxyribonucleic acid extracted from these preparations was about 80% viral and 20% cellular as judged by equilibrium centrifugation in cesium chloride density gradients.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.22.5.914-918.1971

Reference16 articles.

1. High yield isolation of mouse mammary tumor virus;Bond H. E.;J. Nat. Cancer Inst.,1969

2. The nucleic acid of human cytomegalovirus;Crawford L. V.;Virology,1964

3. Density gradient centrifugation of DNA in a fixed angle rotor. A high order of resolution;Flamm W.;Biochem. Biophys. Acta,1966

4. Improved continuous flow centrifugation with banding;Klein G. B.;Nature (London),1966

5. Concentration and purification of herpes-viruses (simplex, cytomegalo, and EB) in a zonal centrifuge;McCombs R. M.;Appl. Microbiol.,1969

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