Author:
Collins J K,Butcher A C,Riegel C A,McGrane V,Blair C D,Teramoto Y A,Winston S
Abstract
Monoclonal antibodies were used to study neutralizing determinants on polypeptides of bovine herpesvirus 1. Two of three monoclonal antibodies which recognized nonoverlapping epitopes on a glycoprotein of 82,000 daltons were found to neutralize. A second group of monoclonal antibodies that individually precipitated five viral glycopolypeptides ranging in size from 102,000 to 55,000 daltons also neutralized. Two monoclonal antibodies which were the most efficient in neutralization recognized a non-glycosylated protein of 115,000 daltons which was the major polypeptide on the virus. A fourth group of monoclonal antibodies precipitated a non-glycosylated polypeptide of 91,000 daltons and several smaller polypeptides, but these antibodies demonstrated only limited neutralizing activity.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference26 articles.
1. Membrane proteins specified by herpes simplex viruses. V. Identification of an Fcbinding glycoprotein;Baucke R. B.;Virology,1979
2. Identification of envelope and nucleocapsid proteins of infectious bovine rhinotracheitis virus by SDS-polyacrylamide gel electrophoresis;Bolton D. C.;Vet. Microbiol.,1983
3. Application of denatured, electrophoretically separated, and immunobilized Iysates of herpes simplex virus-infected cells for detection of monoclonal antibodies and for studies of the properties of viral proteins;Braun D. K.;J. Virol.,1983
4. Recommended standard laboratory techniques for diagnosing infectious bovine rhinotracheitis, bovine virus diarrhea and shipping fever;Carbrey E. A.;Proc. U. S. Anim. Health Assoc.,1971
5. Synthesis, processing, and cell surface expression of Friend and xenotropic murine leukemia virus gp 7O antigens on Friend virus-induced erythroleukemia cell clones;Collins J. K.;J. Immunol.,1980
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