Restriction Maps of Five Autographa californica M NPV Variants, Trichoplusia ni M NPV, and Galleria mellonella M NPV DNAs with Endonucleases Sma I, Kpn I, Bam HI, Sac I, Xho I, and Eco RI

Abstract

The restriction sites of Autographa californica nuclear polyhedrosis virus (Ac M NPV) E2 DNA were mapped for the endonucleases Sma I, Kpn I, Bam HI, Sac I, Xho I, and Eco RI. The restriction maps of four other Ac M NPV variants, Trichoplusia ni (Tn M NPV), and Galleria mellonella (Gm M NPV) genomes were determined and compared to the endonuclease cleavage maps of Ac M NPV E2 DNA. The viral structural polypeptides of Ac M NPV variants S3, E2, S1, M3, and R9 were the same when analyzed by polyacrylamide gel electrophoresis. The major structural polypeptides of Gm M NPV and Tn M NPV had the same pattern in polyacrylamide gels as did Ac M NPV structural polypeptides. Gm M NPV and Tn M NPV had several minor structural protein differences as compared with Ac M NPV. Ac M NPV variants, Tn M NPV, and Gm M NPV were distinct but with very similar genomes and protein structures.