Affiliation:
1. Departments of Medicine, Pathology and Pediatrics, University of Chicago, Chicago, Illinois 60637
Abstract
The multiplication of a new, ether-sensitive, ribonucleic acid virus, 229E, isolated from the human respiratory tract, has been studied in cultures of WI-38 human diploid cells. In thin sections of these cells examined with the electron microscope, particles appeared in vesicles in the cytoplasm of cells at a time corresponding to the initial increase in infectious virus. Antigen was also detected in the cytoplasm of cells by the immunofluorescent technique. Extracellular particles of similar morphology were prominent soon after. These events preceded a detectable cytopathic effect. Later, an electron-dense particle appeared within vacuoles in the cytoplasm but was never found extracellularly. Its role in virus development is not known. Complement-fixing antigen developed along with the increase in infectious virus.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference10 articles.
1. Growth and serological characteristics of respiratory syncytial virus;BENNETT C. R.;J. Infect. Diseases,1962
2. The detection and study of tumor viruses with the electron microscope;BERNHARD W.;Cancer Res.,1960
3. The structure of infectious bronchitis virus;BERRY D. M.;Virology,1964
4. A new virus isolated from the human respiratory tract;HAMRE D.;Proc. Soc. Exptl. Biol. Med.,1966
5. The serial cultivation of human diploid cell strains;HAYFLICK L.;Exptl. Cell Res.,1961
Cited by
59 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献