Affiliation:
1. Department of Microbiology, University of Illinois, Urbana, Illinois 61801
Abstract
The products of an in vitro synthesis with the Qβ replicase purified from
Escherichia coli
infected by the ribonucleic acid (RNA) bacteriophage Qβ were examined by sucrose gradient centrifugation and polyacrylamide gel electrophoresis. It was found that, in contrast to sucrose gradients, gel electrophoresis clearly resolved four classes in the newly synthesized RNA. The product was found to contain mature Qβ-RNA and small-molecular-weight RNA. In addition, two species were identified which corresponded to the structures found in vivo by Francke and Hofschneider and by Franklin. All of the participants implicated in RNA replication by in vivo studies have now been synthesized in vitro and isolated from the reaction mixture.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
13 articles.
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