Molecular Methods for the Epidemiological Typing of Salmonella enterica Serotype Typhi from Hong Kong and Vietnam

Author:

Ling J. M.1,Lo N. W. S.1,Ho Y. M.2,Kam K. M.3,Hoa Nguyen Thi Tuyet4,Phi Le Thi4,Cheng A. F.1

Affiliation:

1. Department of Microbiology, The Chinese University of Hong Kong, The Prince of Wales Hospital, Shatin, New Territories,1

2. Department of Microbiology, The University of Hong Kong, Queen Mary Hospital,2 and

3. Institute of Pathology, Sai Ying Pun Hospital, Sai Ying Pun,3 Hong Kong SAR, China, and

4. Department of Microbiology, Centre for Tropical Diseases, Cho Quan Hospital, Ho Chi Minh City, Vietnam4

Abstract

ABSTRACT A total of 217 and 73 strains of Salmonella enterica serotype Typhi isolated from 1985 to 1997 in Hong Kong and in 2 months of 1989 and 1990 in Vietnam, respectively, were studied. These isolates were typed by plasmid profile analysis, plasmid fingerprinting, ribotyping with Pst I, and total DNA fingerprinting with Nar I. There appeared to be no major outbreak of typhoid fever in Hong Kong during the study period since there was considerable heterogeneity among the isolates. Isolates from Hong Kong were different from those from Vietnam. Thirty-seven percent of Vietnamese isolates belonged to two predominant clones, with the rest being heterogeneous in nature. Total DNA fingerprinting supplemented with ribotyping could be a reliable and rapid method for epidemiological typing of S. enterica serotype Typhi.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference35 articles.

1. Ribosomal RNA Gene Restriction Patterns Provide Increased Sensitivity for Typing Salmonella typhi Strains

2. The characterization of plasmids in the enterobacteria;Anderson E. S.;J. Hyg.,1974

3. Arbeit R. D. Laboratory procedures for the epidemiologic analysis of microorganisms Manual of clinical microbiology 7th ed. Murray P. R. Baron E. J. Pfaller M. A. Tenover F. C. Yolken R. H. 1999 116 137 American Society for Microbiology Washington D.C.

4. Balows A. Hausler W. J. Jr. Herrmann K. L. Isenberg H. D. Shadomy H. J. Manual of clinical microbiology 5th ed. 1991 American Society for Microbiology Washington D.C.

5. To the question of stability of Salmonella typhi phage types;Borecka J.;J. Hyg. Epidemiol. Microbiol. Immunol.,1973

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