Species-Specific Identification of Campylobacters by PCR-Restriction Fragment Length Polymorphism and PCR Targeting of the Gyrase B Gene-Reference-Cited by-同舟云学术

Species-Specific Identification of Campylobacters by PCR-Restriction Fragment Length Polymorphism and PCR Targeting of the Gyrase B Gene

Published:2008-04-15 Issue:8 Volume:74 Page:2529-2533
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Kawasaki Susumu¹,Fratamico Pina M.²,Wesley Irene V.³,Kawamoto Shinichi¹

Affiliation:

1. National Food Research Institute, Food Hygiene Team, 2-1-12 Kannondai, Tsukuba 305-8642, Japan

2. United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, Pennsylvania 19038

3. United States Department of Agriculture, Agricultural Research Service, National Animal Disease Center, 2300 Dayton Roa, Ames, Iowa 50010

Abstract

ABSTRACT PCR-restriction fragment length polymorphism (RFLP) analysis of a 960-bp fragment of the Campylobacter gyrB gene with either DdeI or XspI restriction enzymes generated unique digestion patterns for 12 different Campylobacter species. In addition, PCR assays using species-specific primer sets targeting gyrB were specific for the respective Campylobacter species. Therefore, PCR-RFLP analysis and species-specific PCR assays based on the gyrB gene provide valuable tools for rapid and unambiguous identification of the majority of Campylobacter species.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00975-07

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