Affiliation:
1. Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan
Abstract
ABSTRACT
The dehalorespiring
Desulfitobacterium hafniense
strain Y51 efficiently dechlorinates tetrachloroethene (PCE) to
cis
-1,2-dichloroethene (
cis
-DCE) via trichloroethene by PceA reductive dehalogenase encoded by the
pceA
gene. In a previous study, we found that the significant growth inhibition of strain Y51 occurred in the presence of commercial
cis
-DCE. In this study, it turned out that the growth inhibition was caused by chloroform (CF) contamination of
cis
-DCE. Interestingly, CF did not affect the growth of PCE-nondechlorinating SD (small deletion) and LD (large deletion) variants, where the former fails to transcribe the
pceABC
genes caused by a deletion of the promoter and the latter lost the entire
pceABCT
gene cluster. Therefore, PCE-nondechlorinating variants, mostly LD variant, became predominant, and dechlorination activity was significantly reduced in the presence of CF. Moreover, such a growth inhibitory effect was also observed in the presence of carbon tetrachloride at 1 μM, but not carbon dichloride even at 1 mM.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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