A simple and sensitive DNA hybridization assay used for the routine diagnosis of human parvovirus B19 infection-Reference-Cited by-同舟云学术

A simple and sensitive DNA hybridization assay used for the routine diagnosis of human parvovirus B19 infection

Published:1995-09 Issue:9 Volume:33 Page:2473-2475
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Hicks K E¹,Beard S¹,Cohen B J¹,Clewley J P¹

Affiliation:

1. Virus Reference Division, Central Public Health Laboratory, London, United Kingdom.

Abstract

A dot blot hybridization assay for parvovirus B19 diagnosis was developed by using a PCR-generated probe, digoxigenin labelling, and chemiluminescence detection. Different labelling techniques and hybridization solutions were evaluated. From this analysis a protocol was devised for routine diagnostic use. The protocol enabled 1 pg of B19 DNA to be detected. The results of applying this method to 8,369 diagnostic samples collected during 1994 and 1995 are given.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.33.9.2473-2475.1995

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